Improved system for construction and analysis of single-copy beta-galactosidase operon fusions in Yersinia enterocolitica.
نویسندگان
چکیده
We report a significantly improved system for studying single-copy lacZ operon fusions in Yersinia enterocolitica: a simple procedure for the stable integration of lacZ operon fusions into the ara locus and a strain with a deletion mutation that abolishes the low level of endogenous beta-galactosidase activity.
منابع مشابه
Improved System for Construction and Analysis of Single-Copy -Galactosidase Operon Fusions in Yersinia enterocolitica
Yersinia enterocolitica is a cause of foodborne human gastroenteritis and a favored model organism for studying bacterial virulence (2). Y. enterocolitica virulence gene regulation is studied in many laboratories. This is especially true for the genes encoding the Ysc type III secretion system, which Y. enterocolitica shares in common with the other pathogenic Yersinia species, Y. pestis and Y....
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 71 9 شماره
صفحات -
تاریخ انتشار 2005